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fpbO [2019-05-31 15:18:22]
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fpbO [2019-05-31 15:18:22]

petrobactin (3.4-catecholate siderophore) [SW|ABC transporter] (permease)
locus
BSU03810
pI
9.73
mw
35.32 kDa
protein length
315 aa Sequence Blast
gene length
948 bp Sequence Blast
function
acquisition of iron
product
petrobactin [SW|ABC transporter] (permease)
essential
no
synonyms
yclO
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    433,315 → 434,262

    The protein

    Catalyzed reaction/ biological activity

  • uptake of the siderophore petrobactin [Pubmed|19955416]

    • Protein family

  • FecCD subfamily (according to Swiss-Prot)

    • [SW|Localization]

  • cell membrane [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|552D4C42DCEA4625000160D2625711C61AB11661|fpbN]-[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]-[gene|473628F86C18FE9957841F3C8B45242C90CB341D|fpbP]-[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]
    description
    [Pubmed|19955416]

    regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]
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    Biological materials

    Mutant

  • MGNA-C066 (yclO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2064 NBRP B. subtilis, Japan]
  • BKE03810 (Δ[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AACGAGCAAAGCTATTTTCA, downstream forward: _UP4_TTGCTGTTAAAGGAGAATAA
  • BKK03810 (Δ[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AACGAGCAAAGCTATTTTCA, downstream forward: _UP4_TTGCTGTTAAAGGAGAATAA

    • References

  • 10092453,19955416,12354229,29133393